X染色体の不活性化

三毛猫の色分けはX染色体の不活性化の目に見える例である。毛色の「黒色」と「茶色」はX染色体上の対立遺伝子によって決定される。X染色体の不活性化により2種類の遺伝子のうちの不活性化されなかった片方だけが発現する。

X染色体の不活性化ライオニゼイション、Lyonization)とは哺乳類メスが持つ2つのX染色体のうちの1つが不活性化されるプロセスをいう。X染色体の不活性化は片方のX染色体が抑圧性のヘテロクロマチンにより取り囲まれることにより起こる。X染色体の不活性化はオスがX染色体を1つしか持たないのに対して、メスがX染色体を2つ持つことによりX染色体由来の遺伝子産物X chromosome gene product)を2倍生じることがないように起こる。(遺伝子量補償参照)どちらのX染色体が不活性化されるかはネズミ人間のような高等哺乳類higher mammals)においてはランダムであるが、いったん不活性化が起こるとその細胞においては変化しない。これに対してフクロネズミにおいては父親由来のX染色体が選択的に不活性化される。

歴史

Mary Lyon proposed the random inactivation of one female X chromosome in 1961年 to explain the mottled phenotype of female mice ヘテロ接合型 for coat color 遺伝子. The Lyon hypothesis also accounted for the findings that one copy of the X chromosome in female cells was highly condensed, and that mice with only one copy of the X chromosome developed as fertile females.

メカニズム

タイミング

All mouse cells undergo an early, imprinted inactivation of the paternally-derived X chromosome in four-cell stage . The 胚体外組織extraembryonic tissue) (which give rise to the 胎盤 and other tissues supporting the embryo) retain this early imprinted inactivation, and thus only the maternal X chromosome is active in these tissues.

In the early blastocyst, this initial, imprinted X-inactivation is reversed in the cells of the inner cell mass (which give rise to the embryo), and in these cells both X chromosomes become active again. Each of these cells then independently randomly inactivates one copy of the X chromosome. This inactivation event is irreversible during the lifetime of the cell, so all the descendants of a cell which inactivated a particular X chromosome will also inactivate that same chromosome. This leads to mosaicism if a female is ヘテロ接合型 for a X-linked gene, which can be observed in the coloration of 三毛猫.

X染色体の不活性化はメスの生殖細胞生成の過程(germline)では解除される。したがって、全てののX染色体は活性を持つ。

不活性化するX染色体の選択

正常なメスは2つのX染色体を持ち、1つの細胞において1つのX染色体(Xaと呼ぶ)は活性を持ち、1つは不活性(Xiと呼ぶ)になる。 そして、過剰なX染色体を持つ個体に関する研究によると、2つを超えるX染色体を持つ細胞においては、そのうちの1つだけがXaとなり、残りのX染色体は不活性化されることが分かっている。 このことは、メスのX染色体のデフォルトの状態が不活性であり、1つのX染色体だけが活性を持つように選択されることを示している。

It is hypothesized that there is an autosomally-encoded 'blocking factor' which binds to the X chromosome and prevents its inactivation. The model postulates that there is limiting blocking factor, so once the available blocking factor molecule binds to one X chromosome the remaining X chromosome(s) are not protected from inactivation. This model is supported by the existence of a single Xa in cells with many X chromosomes and by the existence of two active X chromosomes in cell lines with twice the normal number of autosomes.

X染色体上のX染色体不活性化中心 (X Inacivation Center, XIC)と呼ばれる遺伝子配列が、X染色体の不活性化を制御する。 The hypothetical blocking factor is predicted to bind to sequences within the XIC.

染色体のコンポーネント

X染色体上にXICの存在することがX染色体の不活性化が起こるための必要十分条件である。XICが常染色体上に転座Chromosomal translocation)した場合、その常染色体が不活性化され、XICを失ったX染色体は不活性化されない。

XICはXistとTsixの2つの翻訳されないリボ核酸を含み、これらがX染色体の不活性化に関係する。XICはさらに既知および未知のレギュレイトリープロテインregulatory protein)との結合部位を含む。

Xist RNA と Tsix RNA

The Xist gene encodes a large RNA which is not believed to encode a 蛋白質. The Xist RNA is the major effector of X-inactivation. The inactive X chromosome is coated by Xist RNA, whereas the Xa is not. The Xist gene is the only gene which is expressed from the Xi but not from the Xa. X chromosomes which lack the Xist gene cannot be inactivated. Artificially placing and expressing the Xist gene on another chromosome leads to silencing of that chromosome.

Prior to inactivation, both X chromosomes weakly express Xist RNA from the Xist gene. During the inactivation process, the future Xa ceases to express Xist, whereas the future Xi dramatically increases Xist RNA production. On the future Xi, the Xist RNA progressively coats the chromosome, spreading out from the XIC; the Xist RNA does not localize to the Xa. The silencing of genes along the Xi occurs soon after coating by Xist RNA.

Like Xist, the Tsix gene encodes a large RNA which is not believed to encode a protein. The Tsix RNA is transcribed antisense to Xist, meaning that the Tsix gene overlaps the Xist gene and is transcribed on the opposite strand of DNA from the Xist gene. Tsix is a negative regulator of Xist; X chromosomes lacking Tsix expression (and thus having high levels of Xist transcription) are inactivated much more frequently than normal chromosomes.

Like Xist, prior to inactivation, both X chromosomes weakly express Tsix RNA from the Tsix gene. Upon the onset of X-inactivation, the future Xi ceases to express Tsix RNA (and increases Xist expression), whereas Xa continues to express Tsix for several days.

不活性化

活性のX染色体上の遺伝子と異なり、不活発なX染色体上の大部分の遺伝子は発現しない。This is due to the silencing of the Xi by repressive ヘテロクロマチン, which coats the Xi DNA and prevents the expression of most genes.

Compared to the Xa, the Xi has high levels of DNA methylation, low levels of histone acetylation, low levels of histone H3 lysine-4 methylation, and high levels of histone H3 lysine-9 methylation, all of which are associated with gene silencing. Additionally, a histone variant called macroH2A is exclusively found on ヌクレオソームs along the Xi.

バー小体

DNA packaged in heterochromatin, such as the Xi, is more condensed than DNA packaged in ユークロマチン, such as the Xa. The inactive X forms a discrete body within the nucleus called a バー小体. The Barr body is generally located on the periphery of the 細胞核, is late DNA複製 within the 細胞周期, and, as it contains the Xi, contains heterochromatin modifications and the Xist RNA.

不活性化されたX染色体上の遺伝子の発現

A fraction of the genes along the X chromosome escape inactivation on the Xi. The Xist gene is expressed at high levels on the Xi and is not expressed on the Xa. Other genes are expressed equally from the Xa and Xi; mice contain few genes which escape silencing whereas up to a quarter of human X chromosome genes are expressed from the Xi. Many of these genes occur in clusters.

Many of the genes which escape inactivation are present along regions of the X chromosome which, unlike the majority of the X chromosome, contain genes also present on the Y染色体. These regions are termed pseudoautosomal regions, as individuals of either sex will receive two copies of every gene in these regions (like an 常染色体), unlike the majority of genes along the sex chromosomes. Since individuals of either sex will receive two copies of every gene in a pseudoautosomal region, no dosage compensation is needed for females, so it is postulated that these regions of DNA have evolved mechanisms to escape X-inactivation. The genes of pseudoautosomal regions of the Xi do not have the typical modifications of the Xi and have little Xist RNA bound.

The existence of genes along the inactive X which are not silenced explains the defects in humans with abnormal numbers of the X chromosome, such as ターナー症候群 (X0) or クラインフェルター症候群 (XXY). Theoretically, X-inactivation should eliminate the differences in gene dosage between affected individuals and individuals with a normal chromosome complement, but in affected individuals the dosage of these non-silenced genes will differ as they escape X-inactivation.

関連項目

参考文献

  • Carrel L, Willard H (2005). "X-inactivation profile reveals extensive variability in X-linked gene expression in females". Nature 434 (7031): 400-4. PMID 15772666.
  • Chow J, Yen Z, Ziesche S, Brown C (2005). "Silencing of the mammalian X chromosome". Annu Rev Genomics Hum Genet 6: 69-92. PMID 16124854.
  • Lyon M (2003). "The Lyon and the LINE hypothesis". Semin Cell Dev Biol 14 (6): 313-8. PMID 15015738.
  • Okamoto I, Otte A, Allis C, Reinberg D, Heard E (2004). "Epigenetic dynamics of imprinted X inactivation during early mouse development". Science 303 (5658): 644-9. PMID 14671313.
  • Plath K, Mlynarczyk-Evans S, Nusinow D, Panning B. "Xist RNA and the mechanism of X chromosome inactivation". Annu Rev Genet 36: 233-78. PMID 12429693.
  • Lyon M (1961). "Gene action in the X-chromosome of the mouse (Mus musculus L.)". Nature 190: 372-3. PMID 13764598
  • X-inactivation as a possible cause for autoimmunity